| Congresso Brasileiro de Microbiologia 2023 | Resumo: 482-1 | ||||
Resumo:The genus Trichosporon causes superficial and systemic mycoses and colonizes the human and pigeon microbiota. Due to the presence of this yast in pigeon feces at sites of anthropic activity, which can colonize a human host, we aimed to identify yeasts of the genus Trichosporon isolated from pigeon feces (PF) and human feces (HF), and to investigate the production of enzymes hydrolytics and their Minimum Inhibitory Concentration (MIC) against fluconazole; and finally, compare the results obtained between the Trichosporon strains isolated from PF with those from HF. We analyzed 15 samples, 7 PF and 8 HF. The samples were identified phenotypically using the classical method, and presumptively, with CHROMAgar Candida. CIM was performed according to CLSI. Phospholipase (FL), proteinase (PL), and lipase (LP) tests were performed according to Price et al., 1982; Ruchel et al., 1982; and Kothavade; Panthaki, 1998, respectively. All (7/100%) PF samples were identified as T. asahii; six (6/75%) in HF, which presented another 2 species (T. brassicae and T. aquatile, 12.5% each). All (100%) strains identified as T. asahii showed powdery blue color on CHROMAgar, while T. brassicae and T. aquatile, had powdery lilac and dark blue colors, respectively. The PF showed Absent (A) activity for FL and PL, and Moderate (M; with 0.5 to 0.68 mm) for LP, in 100% of the samples. Of the eight HF strains, 7 (87.5%) including T. brassicae and T. aquatile were A from PL, 6 (75%) from FL, and 6 M (75%) from LP; one (12.5%) T. asahii strain had M (0.65mm). T. aquatile presented F (0.36mm) of LP and T. brassicae M (0.56mm) of FL. In the reading of the 24-hour MIC PF strains, five (71.42%; 8 ug/mL) were sensitive (S) to fluconazole, one (14.29%) dose-dependent susceptibility (SDD) and one (14, 29%; 128 ug/ml) resistant (R); the 48-hour reading, 3 (37.5%; 8 ug/mL) of the S strains, 3 (37.5%) SDD and 2 (25%; 32 and 128 ug/mL) R. HF of the 24-hour MIC, five (62.5%; 2, 4 and 8 ug/mL) were S to fluconazole, one (12.5%) SDD and 2 (25%; 32 and 64 ug/mL) R; At the 48-hour reading, 3 (37.58 ug/ml) were S, 3 (37.5%) SDD and 2 (25%; 32 and 128 ug/ml) R. The 2 strains of T. asahii isolated from PF that were R, showed the same reading at 24 and 48 hours and LP M activity (0.50 to 0.51mm), while in the 2 R strains of HF, T. brassicae showed 64 and 128 ug/mL at 24 and 48 hours, respectively, T. asahii was 32 ug/ml for both readings. The strains R, T. brassicae, and T. asahii showed M of FL (0.56mm) and M of FL (0.65mm), respectively. We conclude that due to anthropic activity in places where there is PF, there is the possibility of human acquisition of strains highly resistant to fluconazole; and those strains isolated from the human microbiota present factors associated with virulence concomitantly with resistance to fluconazole. The predominant species in HF was the same isolated in PF, and according to the literature, it is the most isolated species in systemic infections in humans. Such facts corroborate the literature data that report systemic infections of endogenous origin by this yeast, and the importance of knowing the environmental microbiota that surrounds us. Palavras-chave: Factors associated with virulence, MIC, Fluconazole, Feces Agência de fomento:UNIVERSIDADE FEDERAL DE ALFENAS - MG | |||||